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The Yale Web Style Guide is a widely-used comprehensive style guide.

WAI Website

The WAI Web Content Accessibility Guidelines 1.0 describe how to make Web pages more accessible to people with disabilities. A set of checkpoints and techniques are extremely valuable. WCAG 2.0

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I like this book a lot because it begins with easier translations (from English) and gradually builds up to harder ones (e.g., Asian languages, right-to-left languages).

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I ordered this book immediately because Jim Thatcher is my guide-of-choice to accessibility, with his keen eye for practical information. He is great at helping people build accessible applications.

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Rosenfeld and Morville apply library science to Web site design and a readable and highly applicable book. They started the Argus Center for Information Architecture.

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King's previous book with a similar name focused on an overlooked but critical aspect of website usability: response time. This new book has more of a focus on "conversion" (getting users to where they want to get), but there is still material on response time. A web site at websiteoptimization.com provides chanpter summaries, figures, links to resources, and more.

http://usability.gov/guidelines/.

As the name implies, these guidelines are based on the best available research.